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KMID : 1098420050130030105
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Korean Journal of Medicinal Crop Science
2005 Volume.13 No. 3 p.105 ~ p.108
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Adventitious Bud Induction and Plant Regeneration from Cotyledon Explants of Camellia japonica L
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Kim Kwang-Soo
Hwang Sung-Jin
Pyo Byoung-Sik
Kim Sun-Min
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Abstract
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Culture conditions for plant regeneration of Camellia japonica were achieved by organogenesis in explants of cotyledon. Seed cotyledons were cultured on MS medium containing various auxin, 2,4-D or NAA and cytokinins BA. The adventitious shoot buds were efficiently formed without embryogenesis on the basal region of cotyledon cultured on MS medium supplemented with 0.1;{mu}g/m{ell} 2.4-D and 1;{mu}g/m{ell} BA. Seed cotyledons could be used as a source of explants in experiments of genetic transformation of the genotypes evaluated for improving the efficiency of production of transgenic Camellia plants.
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KEYWORD
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Camellia japonica L. seed cotyledons, organogenesis, plant regeneration
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